Composite

Part:BBa_K4468008:Design

Designed by: Zhichao Li   Group: iGEM22_HUST-China   (2022-09-30)


T7-PmrA-T7-PmrB-T7 Terminator-PmrC-Oprf-Sitag-T7 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 749


Design Notes

nothing


Source

T7 promoter and T7 terminator are from Bacteriophage T7.
PmrA, PmrB and PmrC are from Salmonella bongori.
Oprf is from P. aeruginosa. Sitag is from Escherichia coli.

References